薄层色谱法检测苏丹红
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(1. 河北科技大学化学与制药工程学院,河北石家庄 050018;2. 石家庄大为生物技术有限公司,河北石家庄 050091)

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窦 红(1982-),女,河北石家庄人,硕士研究生,主要从事食品中有毒、有害物质快速检测技术方面的研究

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Fast detection of Sudan Red by thin layer chromatography
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(1.College of Chemical and Pharmaceutical Engineering,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China;2. David Biotechnology Company Limited, Shijiazhuang Hebei 050091,China)

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    摘要:

    探讨用薄层色谱法检测食品中苏丹红色素的可行性,并对高效硅胶G板及聚酰胺薄层板分离苏丹红色素的结果进行比较,发现高效硅胶G板较聚酰胺薄层板分离苏丹红色素更具有优势,其检测灵敏度高:0.3mg/ kg;分析速度快:用聚酰胺薄层板分析一般需要40min,而高效硅胶G板只需5~10min;分离清晰:4种苏丹红色素与辣椒色素等干扰色素能够完全分离。该方法适用于基层机构在现场快速检测食品中非法添加的苏丹红色素。

    Abstract:

    This paper investigated the feasibility of detecting Sudan Red in foods with thin layer chromatography, and compared the result of Sudan Red separation with high-effect silica gel G plates (HPSGG) and the result of it with polyamide plates. It is found that HPSGG has more advantages over polyamide plates in separating Sudan Red. HPSGG gives high detection sensitivity, up to 0.3mg/ kg, fast analysis and clear separation. Polyamide plates needs 40minutes while high-effect silica gel G plates needs only 5~10minutes. The Sudan pigments and other interference pigments such as pepper pigments,can be completely separated. The method can be applied to rapidly detect Sudan Red illegally added in foods such as chilli products and hamburgers.

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窦 红,张晓鑫,高春平.薄层色谱法检测苏丹红[J].河北工业科技,2009,26(2):90-94

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历史
  • 收稿日期:2008-09-11
  • 最后修改日期:2008-11-13
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  • 在线发布日期: 2013-08-16
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